Journal: International journal of molecular sciences

Article Title: Inhibition of the NF-κB Signaling Pathway Alleviates Pyroptosis in Bladder Epithelial Cells and Neurogenic Bladder Fibrosis.

doi: 10.3390/ijms241311160

Figure Lengend Snippet: Figure 6. Bioinformatics analysis of the transcriptome sequencing results of cells. (A,B) Heatmap analysis of IRGs in cellular DEGs. (C–E) Expression levels of the pyroptosis proteins NLRP3 and ASC in SV-HUC-1 cells were detected by immunofluorescence staining (scale bar = 100 µm). (F) SEM showed TGF-β1-induced epithelial cell swelling, cell membrane pore formation, and py- roptotic body generation. Scale bar = 5 µm. (G) TEM showed cell membrane disruption, disruption of organelle structures such as mitochondria and the endoplasmic reticulum, and vacuolization. Scale bar = 2 µm and scale bar = 500 nm. (H) Protein expression levels of NLRP3, GSDMD, ASC, and IL1-β were detected by Western blotting in vitro. The data are presented as the mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The pyroptosis inhibitor MCC950 (10 μM, MCE, HY-12815A) was used to inhibit pyroptosis in SV-HUC-1 cells for 2 h before TGF-β1 stimulation [37,38].

Techniques: Sequencing, Expressing, Staining, Membrane, Disruption, Western Blot, In Vitro

Journal: Frontiers in Stroke

Article Title: NLRP3 inflammasome inhibition protects against intracranial aneurysm rupture and alters the phenotype of infiltrating macrophages

doi: 10.3389/fstro.2023.1202137

Figure Lengend Snippet: MCC950 treatment reduces the aneurysm rupture rate and extends symptom-free survival. (A) Aneurysm formation rates, (B) Aneurysm rupture rate, * p < 0.05 by Fisher's exact test. (C) Cumulative symptom-free survival, and (D) Mean arterial pressure, p = n.s. at all-time points.

Article Snippet: MCC950 was obtained from Selleckchem (Catalog No.S7809, Houston, TX) and diluted in 0.9% NaCl in water.

Techniques:

Journal: Frontiers in Stroke

Article Title: NLRP3 inflammasome inhibition protects against intracranial aneurysm rupture and alters the phenotype of infiltrating macrophages

doi: 10.3389/fstro.2023.1202137

Figure Lengend Snippet: MCC950 treatment alters macrophage phenotype ratio in cerebral vasculature after aneurysm induction. (A) Representative images of M1-type (top) and M2-type (bottom) macrophages in saline-treated (left) and MCC950-treated (right) mice, scale bar = 50 μm. (B) Ratio of M1:M2 macrophage phenotypes in the vessel wall, * p < 0.05, n = 5 each. (C) Total number of F4/80 + cell per high-powered field, n = 5 each. All plots presented as median + 95% CI.

Article Snippet: MCC950 was obtained from Selleckchem (Catalog No.S7809, Houston, TX) and diluted in 0.9% NaCl in water.

Techniques: Saline

Journal: Indian Journal of Pharmaceutical Sciences

Article Title: Effects of Propofol Anesthesia on Pyroptosis and the Nod-Like Receptor Protein 3/Caspase-1 Pathway in the Neonatal Rat Hippocampus

doi: 10.36468/pharmaceutical-sciences.1098

Figure Lengend Snippet: Fig. 2: The protein expression of NLRP3, ASC and caspase-1 in the hippocampal tissues of rats in each group

Article Snippet: Main reagents: Propofol (Cat No.: T1300) was purchased from target molecule Corp, United States of America (USA); the NLRP3 inhibitor MCC950 (Cat No: T6887) was purchased from TargetMol, USA; Transferase dUTP Nick End Labeling (TUNEL) kit (Cat No.: AT2190-50T) was purchased from Shanghai ACMEC Biochemical Technology Co., Ltd.; Interleukin-1 beta (IL-1β), Interleukin-18 (IL-18) detection kit (Cat No.: YAD1545 and YAD1758) was purchased from Beijing Jiehui Bogao Biotechnology Co., Ltd.; Bicinchoninic Acid (BCA) protein assay kit (Cat No.: 701780-480) was purchased from Cayman, USA; primary antibodies NLRP3 antibody, ASC antibody, caspase-1 antibody, Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody and secondary antibodies Immunoglobulin G (IgG) antibody (Cat No.: ab263899, ab175449, ab138483, ab59164 and ab6721) were purchased from Abcam.

Techniques: Expressing